Could Anti-Virus or security software interfere with Wine and cause this error?

Yes, some anti-virus or security software might flag Wine or its components as suspicious, preventing it from functioning correctly. This can lead to import errors if the Python script relies on Wine. Try temporarily disabling the anti-virus software (at your own risk and only for testing purposes) to see if it resolves the issue. If it does, configure the anti-virus to exclude Wine-related executables and directories from scanning.